Effect of lipoteichoic acid of Bifidobacterium on survivin and its regulatory genes.
- Author:
Yue WANG
1
;
Tao MAI
;
Ming-fang LIU
;
Shu-hui CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Bifidobacterium; chemistry; Blotting, Western; Cell Line, Tumor; Colonic Neoplasms; genetics; metabolism; pathology; Dose-Response Relationship, Drug; Down-Regulation; drug effects; Humans; Inhibitor of Apoptosis Proteins; Lipopolysaccharides; isolation & purification; pharmacology; Microtubule-Associated Proteins; genetics; metabolism; Neoplasm Proteins; genetics; metabolism; PTEN Phosphohydrolase; metabolism; Phosphatidylinositol 3-Kinases; metabolism; Proto-Oncogene Proteins c-akt; metabolism; RNA, Messenger; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; drug effects; Teichoic Acids; isolation & purification; pharmacology; Tumor Suppressor Protein p53; metabolism
- From: Chinese Journal of Oncology 2007;29(5):325-328
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThe aim of this study is designed to explore the anti-tumor effect of lipoteichoic acid (LTA) of Bifidobacterium on the expression of survivin in colon cancer LoVo cells and its possible regulatory mechanism.
METHODSThe changes of survivin mRNA and protein in LoVo cells treated with LTA of Bifidobacterium were detected by RT-PCR and Western blot. Meanwhile, the expressions of pAKT (the key protein kinase in P13K/AKT signal transduction pathway), p53 and PTEN were measured by Western blot.
RESULTSThere were overexpressions of survivin mRNA and protein in LoVo cells. After treated with different dose of LTA of Bifidobacterium, the expressions of survivin mRNA and protein were markedly decreased in a dose-dependent manner (P < 0.01). Besides, the activity of pAKT was decreased significantly (P < 0.01) and the expression of p53 and PTEN was increased (P < 0.01).
CONCLUSIONLTA of Bifidobacterium can down-regulate the expression of survivin in LoVo cells through inhibiting the activity of PI3K/AKT signal transduction pathway and up-regulate the expression of p53. Accordingly, the activity of caspases is increased, and apoptosis of LoVo cells occurs ultimately.
