Correlation of Helicobacter pylori infection with the expression of COX-2 and EGFR and VEGF in human gastric carcinoma.
- Author:
Wei-Ming XIAO
1
;
Yan-Bing DING
;
Rui-Hua SHI
;
Wei-Juan GONG
;
Yan XUE
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Blotting, Western; Cyclooxygenase 2; metabolism; Female; Gastric Mucosa; metabolism; Gene Expression Regulation, Neoplastic; Helicobacter Infections; metabolism; microbiology; Helicobacter pylori; Humans; Immunohistochemistry; Male; Middle Aged; Receptor, Epidermal Growth Factor; metabolism; Stomach Neoplasms; metabolism; microbiology; Up-Regulation; Vascular Endothelial Growth Factor A; metabolism
- From: Chinese Journal of Oncology 2008;30(9):668-671
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the correlation of Helicobactor pylori (Hp) infection with the expression of COX-2, EGFR and VEGF in human gastric carcinoma.
METHODSThe expression of COX-2, EGFR and VEGF was detected by immunohistochemistry in samples of 61 gastric cancers and 20 cancer-adjacent tissues. Western blotting was performed in samples of 10 gastric cancers and corresponding cancer-adjacent tissues. Hp infection was detected in 47 patients by fast urea enzyme test and (13)C breath test.
RESULTSThe results of immunohistochemistry showed that the positive expressions of COX-2, EGFR and VEGF in gastric carcinoma were 59.02%, 36.07% and 60.66%, respectively, significantly higher than that in the normal mucosa (25.00%, 0 and 30.00%, respectively). There was a positive correlation between the expression of COX-2, EGFR and VEGF and gastric carcinoma. The expression of COX-2 and EGFR was 75.76% and 45.45% in the gastric carcinomas with Hp infection, significantly higher than that in those without (28.57% and 14.29%). The protein expression of COX-2, EGFR and VEGF detected by Western blot in gastric carcinomas was also significantly higher than that in normal mucosa.
CONCLUSIONCOX-2, EGFR and VEGF are overexpressed in gastric carcinoma, and there is a positive correlation among them. Hp infection may upregulate the expression of COX-2 and EGFR in gastric cancer tissues.
