Clinical application of avidin-biotin ELISA to detect serum hepatoma-specific gamma-glutamyltransferase in patients with primary hepatic cancer.
- Author:
Nian-yue WANG
1
;
Wei ZHAO
;
Dai ZHANG
;
Yong-cheng ZHANG
;
Mu-hao DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Avidin; Biotin; Carcinoma, Hepatocellular; blood; diagnosis; enzymology; Enzyme-Linked Immunosorbent Assay; methods; Female; Humans; Liver Neoplasms; blood; diagnosis; enzymology; Male; Middle Aged; Sensitivity and Specificity; Young Adult; alpha-Fetoproteins; analysis; gamma-Glutamyltransferase; blood
- From: Chinese Journal of Oncology 2009;31(2):114-117
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect serum hepatoma-specific datura stramonium lectin-tightly binding gamma-glutamyl transferase (DSA-GGT) in patients with primary hepatic cancer (PHC) by avidin-biotin ELISA method which was established in our laboratory, and carry on a study of its clinical application.
METHODSTo detect serum DSA-GGT in 45 healthy control subjects, 58 PHC patients and 203 non-PHC patients (including 36 patients with other tumors and 167 patients with benign liver diseases) with the method was established; meanwhile, AFP was detected by ELISA method.
RESULTS38 individuals were DSA-GGT positive in 58 PHC patients, the sensitivity was 65.5%. 18 individuals were DSA-GGT positive in 203 patients without PHC, the specificity was 91.1%. The sensitivity and specificity of AFP in diagnosis of PHC patients was 69.0% and 90.6%, respectively. The sensitivity and specificity of combination of DSA-GGT and AFP was 93.1% and 85.7%, respectively. The average intra-CV and inter-CV of DSA-GGT ELISA was 8.9% and 11.5%, respectively.
CONCLUSIONThe sensitivity and specificity of DSA-GGT ELISA method established in our lab is similar with that of AFP assay and the accuracy is good. Combination of DSA-GGT and AFP may improve the diagnostic sensitivity. The method should be potentially as a new way to improve diagnosis of PHC.
