Growth of breast cancer cells is inhibited in vitro by cyclin E-siRNA.
- Author:
Wen-shan HE
1
;
Tao HUANG
;
Jing-hua REN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibiotics, Antineoplastic; pharmacology; Antimetabolites, Antineoplastic; pharmacology; Antineoplastic Agents, Phytogenic; pharmacology; Breast Neoplasms; metabolism; pathology; Cell Cycle; Cell Line, Tumor; Cell Proliferation; drug effects; Cyclin E; genetics; metabolism; Doxorubicin; pharmacology; Female; Fluorouracil; pharmacology; Genetic Vectors; Humans; Male; Mice; Mice, Inbred ICR; Mice, Nude; Neoplasm Transplantation; Paclitaxel; pharmacology; Plasmids; RNA Interference; RNA, Messenger; metabolism; RNA, Small Interfering; genetics; Transfection; Tumor Burden
- From: Chinese Journal of Oncology 2009;31(3):170-173
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes of biological characteristics of breast cancer cell line by cyclin E expression.
METHODSHuman breast cancer cell line MCF-7 was transfected with cyclin E siRNA vector pEGFP/CCNE2. siRNA-induced silencing of cyclin E was determined by RT-PCR at RNA level and Western blot at protein level. The proliferation of MCF-7 cells and their sensitivity to chemotherapy was measured by CCK-8 assay. The cells were examined by FCM. The cell line was injected into nude mice and the tumor size was measured.
RESULTSThe expression of cyclin E was inhibited in the MCF-7 cells. The relative expression level of cyclin E mRNA was 0.23 +/- 0.05, and that of cyclin E protein was 0.24 +/- 0.05. The cell growth was inhibited by 68.56% +/- 0.08%, and their sensitivity to chemotherapy was increased. Most cells were blocked at G(1) (77.38%), their tumorigenic ability in nude mice was reduced, and the size of tumor formed in mice of the experimental group was decreased than that of controls.
CONCLUSIONInhibition of cyclin E expression in breast cancer cells can block their cell cycle at G(1) phase, reduce their cell growth, differentiation and proliferation, and increase their sensitivity to chemotherapy.
