Expression of transcription factor T-bet and GATA-3 in peripheral blood mononuclear cells and its correlation with immune status in esophageal cancer patients.
- Author:
De-yu CHEN
1
;
Ya-wei SHI
;
Ye HUA
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Enzyme-Linked Immunosorbent Assay; Esophageal Neoplasms; immunology; metabolism; pathology; Female; GATA3 Transcription Factor; genetics; metabolism; Humans; Interferon-gamma; blood; Interleukin-4; blood; Leukocytes, Mononuclear; metabolism; Male; Middle Aged; Neoplasm Staging; RNA, Messenger; metabolism; Reverse Transcriptase Polymerase Chain Reaction; T-Box Domain Proteins; genetics; metabolism; Th1 Cells; metabolism; Th2 Cells; metabolism
- From: Chinese Journal of Oncology 2009;31(3):189-191
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the expression of transcription factors (TF) T-bet and GATA-3 mRNA in peripheral blood mononuclear cells and its correlation with immune status in esophageal cancer patients.
METHODSSixty patients were divided into two groups according to the clinical data: group A consisting of stage I and II, group B including stage III and IV. The gene expression of T-bet and GATA-3 in 60 esophageal cancer patients and 30 healthy controls was detected by reverse transcription-polymerase chain reaction (RT-PCR). The expression of IFN-gamma and IL-4 was measured by enzyme linked immunosorbent assay (ELISA).
RESULTSExpression of T-bet mRNA in esophageal cancer patients (stage I and II: 0.27 +/- 0.05 ng/L, stage III and IV: 0.12 +/- 0.02 ng/L) was significantly lower than that in the healthy controls (1.35 +/- 0.14 ng/L), but the expression of GATA-3 mRNA in esophageal cancer patients (stage I and II: 0.45 +/- 0.06, stage III and IV: 0.55 +/- 0.03) was significantly higher than that in the healthy controls (0.09 +/- 0.10). The plasma level of Th1 cytokine IFN-gamma in the patients [stage I and II: (12.12 +/- 1.48) ng/L, stage III and IV: (8.44 +/- 0.90) ng/L] was significantly lower than that in the healthy controls, while the level of Th2 cytokine IL-4 in the patients [stage I and II: (18.64 +/- 0.77) ng/L, stage III and IV: (25.28 +/- 2.02) ng/L] was significantly higher than that in the healthy controls. However, neither in the expression of T-bet and GATA-3, nor in the plasma level of IFN-gamma and IL-4, showed a significant difference between group A and B.
CONCLUSIONIn the peripheral blood of esophageal cancer patients, the expression of T-bet decreased, while GATA-3 increased, Th1/Th2 balance is broken, and the Th2 is dominant. T-bet and GATA-3 play a part role in the regulation of Th1/Th2 balance.
