Application of serum protein markers to distinguish familial adenomatous polyposis (FAP) and sporadic colorectal adenomas.
- Author:
Shan-rong CAI
1
;
Jie-kai YU
;
Wen-zhi JIANG
;
Su-zhan ZHANG
;
Shu ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoma; genetics; metabolism; Adenomatous Polyposis Coli; genetics; metabolism; Adult; Aged; Biomarkers, Tumor; metabolism; Colorectal Neoplasms; genetics; metabolism; Diagnosis, Differential; Female; Gene Expression Profiling; Humans; Male; Middle Aged; Protein Array Analysis; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: Chinese Journal of Oncology 2009;31(3):192-195
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen out specifically-expressed serum protein markers in familial adenomatous polyposis (FAP) and to establish a serum protein fingerprint diagnostic model for distinguishing FAP from sporadic colorectal adenomas.
METHODSSerum samples were collected from 19 FAP cases and 16 sporadic colorectal adenomas with informed consent. Serum protein fingerprint profiles were detected by SELDI-TOF-MS with CM 10 protein chip to screen out FAP adenoma-related serum protein markers, and support vector machine (SVG) technique was used to establish the diagnostic model to distinguish FAP from sporadic colorectal adenomas.
RESULTSSix differently-expressed protein peaks (P < 0.01) were detected. Among them proteins of 5640, 3160, 4180 and 4290 m/z were highly expressed in FAP adenomas, and proteins of 3940 and 3400 m/z were highly expressed in sporadic colorectal adenomas. The accuracy of diagnostic model established with SVG to distinguish FAP adenomas and sporadic colorectal adenomas was 94.7% and 93.7%, respectively.
CONCLUSIONSELDI-TOF-MS can be effectively used to screen out the differentially expressed serum protein markers in FAP adenomas and sporadic colorectal adenomas, and a diagnostic model build by SVG to distinguish them has been successfully established. Therefore, a useful breakthrough point for research on molecular mechanisms of FAP pathogenesis is provided.
