The effect of biomechanical stimulation on osteoblast differentiation of human jaw periosteum-derived stem cells.
10.1186/s40902-017-0104-6
- Author:
Ju Min LEE
1
;
Min Gu KIM
;
June Ho BYUN
;
Gyoo Cheon KIM
;
Jung Hoon RO
;
Dae Seok HWANG
;
Byul Bora CHOI
;
Geun Chul PARK
;
Uk Kyu KIM
Author Information
1. JUM Oral and Maxillofacial Surgery Clinic, Seoul, South Korea.
- Publication Type:Original Article
- Keywords:
Biomechanical stimulation;
Cyclic tension force;
Osteoblast differentiation;
Human jaw periosteum-derived stem cells;
New bioreactor;
Human mesenchymal stem cells
- MeSH:
Alkaline Phosphatase;
Bioreactors;
Calcium;
Humans*;
Jaw*;
Mandible;
Molar, Third;
Osteoblasts*;
Osteocalcin;
Osteonectin;
RNA, Messenger;
Stem Cells*;
Tissue Engineering;
Vascular Endothelial Growth Factor A
- From:Maxillofacial Plastic and Reconstructive Surgery
2017;39(3):7-
- CountryRepublic of Korea
- Language:English
-
Abstract:
BACKGROUND: This study was to investigate the effect of biomechanical stimulation on osteoblast differentiation of human periosteal-derived stem cell using the newly developed bioreactor. METHODS: Human periosteal-derived stem cells were harvested from the mandible during the extraction of an impacted third molar. Using the new bioreactor, 4% cyclic equibiaxial tension force (0.5 Hz) was applied for 2 and 8 h on the stem cells and cultured for 3, 7, and 14 days on the osteogenic medium. Biochemical changes of the osteoblasts after the biomechanical stimulation were investigated. No treatment group was referred to as control group. RESULTS: Alkaline phosphatase (ALP) activity and ALP messenger RNA (mRNA) expression level were higher in the strain group than those in the control group. The osteocalcin and osteonectin mRNA expressions were higher in the strain group compared to those in the control group on days 7 and 14. The vascular endothelial growth factor (VEGF) mRNA expression was higher in the strain group in comparison to that in the control group. Concentration of alizarin red S corresponding to calcium content was higher in the strain group than in the control group. CONCLUSIONS: The study suggests that cyclic tension force could influence the osteoblast differentiation of periosteal-derived stem cells under optimal stimulation condition and the force could be applicable for tissue engineering.
