Expression of antisense thrombin receptor gene inhibits intimal hyperplasia of rat carotid artery after balloon injury.
- Author:
Guofeng REN
1
;
Zongli WANG
;
Yongjun LI
;
Jing YANG
;
Peimao LIU
;
Mingpeng SHE
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carotid Arteries; Hyperplasia; Rats; Receptors, Thrombin; metabolism; Thrombin; pharmacology; Tunica Intima; metabolism
- From: Chinese Journal of Pathology 2002;31(3):231-235
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the mechanism of restenosis after angioplasty and to clarify the effect of thrombin and its receptor on restenosis development.
METHODSBalloon catheter-induced injury was adopted to induce intimal hyperplasia of the carotid arteries in rats. Antisense thrombin receptor (ATR) cDNA was transfected by perfusing recombinant LXSN ATR plasmid/nanoparticle complex into the segment of the injured carotid artery.
RESULTSPCR result showed integration of the recombined gene. Dot blot showed the expression of antisense TR mediated by recombinant LXSN ATR plasmid/nanoparticle complex in the wall of common carotid arteries of the experimental group rats, which enabled to inhibit TR gene expression and intimal hyperplasia of the injured arteries.
CONCLUSIONSThrombin and its receptor play an important role in the formation of neointima after the injury, which provides a potential clue in developing a new approach for prevention and treatment of restenosis after angioplasty.
