Effects of selenium and iodine on the expression of c-fos and c-jun mRNA and their proteins in cultured rat hippocampus neurons.
- Author:
Dongping TIAN
1
;
Min SU
;
Xianying WU
;
Qiaoshan LI
;
Ruiming ZHENG
;
Guangyuan LI
;
Tianbao SONG
;
Xiaohu XU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; DNA-Binding Proteins; metabolism; Hippocampus; metabolism; Iodine; Neurons; metabolism; Proto-Oncogene Proteins c-fos; metabolism; Proto-Oncogene Proteins c-jun; metabolism; RNA, Messenger; metabolism; Rats; Selenium
- From: Chinese Journal of Pathology 2002;31(3):245-249
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the effect of selenium (Se) and iodine (I) and the compound of both on the proto-oncogenes c-fos and c-jun mRNA and their protein expression in the cultured rat hippocampus neurons.
METHODSUsing the technique of serum free hippocampus neuron culture, different doses of Se and I and Se + I compound were added into the medium. The expression of the mRNA of c-fos, c-jun in hippocampus neurons cultured for 1, 3, 5, 7 and 10 d were studied using both in situ hybridization and SABC immunohistochemical technique.
RESULTSBoth Se and I could enhance the expression of c-fos, c-jun mRNA and their proteins, especially the combination of I and Se able to give a remarkable effect on c-jun mRNA expression.
CONCLUSIONSSe and I may effect the expression of both c-fos and c-jun mRNA, especially the c-jun mRNA and its protein of hippocampus neurons, and thus may effect the differentiation and development of neurons.
