The beneficial effect of phVEGF165 transfer on vascular remodelling after balloon injury and its possible mechanisms.
- Author:
Fang WEI
1
;
Qingshan GENG
;
Bin ZHANG
;
Jianzhang FENG
;
Huahuan LIN
;
Zuxun JIANG
;
Xiyong YU
;
Gang ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Angioplasty, Balloon; adverse effects; Animals; Coronary Restenosis; etiology; pathology; Endothelial Growth Factors; genetics; physiology; Intercellular Signaling Peptides and Proteins; genetics; physiology; Lymphokines; genetics; physiology; Male; Matrix Metalloproteinases; metabolism; Rabbits; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors
- From: Chinese Journal of Pathology 2002;31(5):436-439
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of phVEGF165 transfer on vascular remodelling after balloon injury and its possible mechanisms.
METHODS90 New Zealand white rabbits were divided randomly into 3 groups: group I (balloon injury group), group II (pAdtrackCMV group) and group III (pAdtrackCMV-VEGF165 group). All animals were given hypercholesterol diet for 7 days before experiment and continued to receive hypercholesterol diet until being killed. Each group was further divided into five subgroups according to the sacrifice time (3 days, 1, 2, 4 and 8 weeks after transfection). Blood samples and arteries were harvested for further analysis.
RESULTSAt the end of 2 weeks, areas of neointima plus media of group III were smaller than those of group I and II (P < 0.05). The areas under external elastic membrane were larger in group III at 4 weeks and lumen stenosis rates were significantly lower than group I and II (P < 0.05 or 0.01). In group III, VEGF165, metalloproteinases (MMPs) -1, -2, -9 and their tissue inhibitors (TIMPs) 1, 2 could be detected from 3 days after gene transfer and reached the highest level at 2 weeks time and could not be detected by 8 weeks time. In groups I and II, MMP-2 and TIMP-1, -2 could be detected during the whole procedure and the value of TIMP1/MMP1 was significantly higher than in group III (P < 0.01).
CONCLUSIONRemodelling is the main reason for restenosis (RS) after vascular balloon injury. Local pAdtrackCMV-VEGF165 transfer can specifically change the expression of MMPs and facilitate the positive remodelling process, hence, inhibiting restenosis.
