Relationship between mitochondrial DNA instability and interleukin-8 activity in gastric mucosa.
- Author:
Xian-long LING
1
;
Dian-chun FANG
;
Rong-quan WANG
;
Shi-ming YANG
;
Yuan-hui LUO
Author Information
- Publication Type:Journal Article
- MeSH: DNA, Mitochondrial; genetics; Enzyme-Linked Immunosorbent Assay; Gastric Mucosa; metabolism; pathology; Gastritis, Atrophic; genetics; metabolism; Genomic Instability; Humans; Interleukin-8; metabolism; Metaplasia; genetics; metabolism; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Precancerous Conditions; genetics; metabolism; Stomach Neoplasms; genetics; metabolism
- From: Chinese Journal of Pathology 2003;32(1):35-37
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the relationship between mitochondrial DNA instability (mtMSI) and interleukin-8 (IL-8) activity in gastric mucosa of various lesions.
METHODSIL-8 level in gastric mucosa was assayed using ELISA method. The mtMSI was detected by PCR-SSCP techniques.
RESULTSmtMSI was observed in 11 out of 30 (36.7%) gastric cancers, 2 of 15 (13.3%) intestinal metaplasia, 2 of 10 dysplasia and 1 of 10 chronic atrophic gastritis. IL-8 level in mtMSI+ group [(76.8 +/- 3.8) pg/mg] was significantly higher than that in mtMSI- group [(48.3 +/- 3.6) pg/mg, P < 0.05].
CONCLUSIONmtMSI closely correlates with IL-8 level in gastric mucosa and is involved in gastric carcinogenesis.
