Effects of two variants of ING1 expression on tumor cell growth regulation.
- Author:
Jian-ying LIU
1
;
Bing-quan WU
;
Jie ZHENG
;
Jiang-feng YOU
;
Hao-hao ZHONG
;
Jie-liang WANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenocarcinoma; genetics; metabolism; pathology; Alternative Splicing; Breast Neoplasms; genetics; metabolism; pathology; Cell Cycle; Cell Cycle Proteins; Cell Division; Cell Line, Tumor; Cyclin-Dependent Kinase Inhibitor p21; Cyclins; biosynthesis; DNA-Binding Proteins; Genes, Tumor Suppressor; Humans; Inhibitor of Growth Protein 1; Intracellular Signaling Peptides and Proteins; Lung Neoplasms; genetics; metabolism; pathology; Nuclear Proteins; Protein Biosynthesis; Proteins; genetics; Transfection; Tumor Suppressor Protein p53; biosynthesis; Tumor Suppressor Proteins
- From: Chinese Journal of Pathology 2003;32(1):48-51
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study effects of alternative transcripts of ING1 transfection on human cancer cell lines.
METHODSp47/ING1A and p33/ING1B expression vehicles were constructed and introduced into a human breast cancer cell line MCF-7 and a human lung cancer cell line PAa, both expressing wild-type p53 protein. Growth characteristics of the transfectants and potentially related genes were analyzed.
RESULTSThe levels of p47/ING1A and p33/ING1B protein elevated respectively in tumor cells of MCF-7 and PAa after transfected with p47/ING1A and p33/ING1B, and the latter was much higher than that of the former. Ectopic overexpression of p33/ING1B effectively blocked tumor cell growth and arrested cells in the G(0) approximately G(1) phase of the cell cycle (P < 0.01), while p47/ING1A gave no effect on cell growth or cell cycle. Tumor cells overexpressing p33/ING1B contained more p21(WAF1) protein than that of the control cells, with undisturbed p53 protein level.
CONCLUSIONSExpression of two different transcripts of ING1 may have different effects on tumor cell growth. p33/ING1B may cooperate with p53 in stimulating expression of p21(WAF1) gene, thus to arrest cell cycle and to inhibit tumor cell growth. p33/ING1B may be considered to be a candidate as a partner of p53 in gene therapy.
