Specific loss of heterozygosity of chromosome 3p loci in soft tissue leiomyosarcoma.
- Author:
Hui PENG
1
;
Guang-hua YANG
;
Hong BU
;
Li-xin GOU
Author Information
- Publication Type:Journal Article
- MeSH: Abdominal Neoplasms; genetics; pathology; Chromosomes, Human, Pair 3; Extremities; Genes, Tumor Suppressor; Humans; Leiomyosarcoma; genetics; pathology; Loss of Heterozygosity; Microsatellite Repeats; Retroperitoneal Neoplasms; genetics; pathology; Soft Tissue Neoplasms; genetics; pathology
- From:Chinese Journal of Pathology 2003;32(2):124-127
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the loss of heterozygosity (LOH) at 5 loci on chromosome 3p in soft tissue leiomyosarcoma (LMS).
METHODSLOH was detected in 22 cases of LMS using PCR-silver staining targeting 5 microsatellite sites on 3p14.2-pter. Relation between LOH and LMS clinical pathological features was also analyzed.
RESULTSTen of 22 LMS samples showed LOH at more than one locus (45.4%). Among the 5 loci, LOH occurred more frequently at D3s1295 (36.8%) and D3s1289 (10.5%), but absent at D3s1293. No significant difference was found on LOH incidence between different grade, size and location of LMS.
CONCLUSIONSLOH on chromosome 3p14.2-23 region is relatively frequent in LMS. Region around D3s1295 and D3s1289 may harbor tumor suppressor gene relating to LMS.
