Kinetic turbidimetric limulus assay of bacterial endotoxin in the replacement solution of on-line hemodiafiltration.
- Author:
Hai-yan LAO
1
;
Qiu-xiao LIN
;
Min YANG
;
Xin-ling LIANG
;
Wei SHI
;
Hui LI
;
Ju-jin FENG
Author Information
- Publication Type:Journal Article
- MeSH: Endotoxins; analysis; Hemodiafiltration; methods; Hemodialysis Solutions; analysis; Humans; Kinetics; Limulus Test; Nephelometry and Turbidimetry; methods
- From: Journal of Southern Medical University 2006;26(2):242-244
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine bacterial endotoxin in the replacement solution of on-line hemodiafiltration (on-line HDF) using kinetic turbidimetric limulus test.
METHODS AND RESULTSValidation test was performed with the replacement solution of on-line HDF in which quantified standard endotoxin was added. The recovery rates of endotoxin from the replacement solution and its dilutions at 1/5, 1/10, and 1/20 were 58.17%, 106.7%, 99.00% and 98.79%, respectively, suggesting that the optimal dilution was at 1/10. Standard endotoxin was added into the replacement solution of on-line HDF of 3 batches (040408, 040511,040527), and the recovery rates in their dilution at 1/10 were 76.32%, 99.00% and 96.24%, respectively. The standard endotoxin in the working curve was 1.00, 0.125, and 0.0156 Eu/ml (endotoxin unit/ml), and the dilution at 1/10 of the replacement solution is effective to eliminate the interference in limulus test.
CONCLUSIONKinetic turbidimetric limulus test provide a means to detect endotoxin in the replacement solution of on-line HDF.
