Synergism between a siRNA targeted to survivin and 5-FU in inhibiting MCF-7 cell proliferation in vitro.
- Author:
Shu-qun ZHANG
1
;
Shu-hui ZHANG
;
Xin-huan XUE
;
Xi-jing WANG
;
Jian-tao JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Antimetabolites, Antineoplastic; pharmacology; Apoptosis; drug effects; genetics; Cell Line, Tumor; Cell Proliferation; drug effects; Cell Survival; drug effects; genetics; Dose-Response Relationship, Drug; Female; Fluorouracil; pharmacology; Genetic Vectors; genetics; Humans; Inhibitor of Apoptosis Proteins; Microtubule-Associated Proteins; genetics; Neoplasm Proteins; genetics; RNA Interference; RNA, Small Interfering; genetics; Transfection
- From: Journal of Southern Medical University 2006;26(3):251-254
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the role of small interfering RNA (siRNA) targeted to survivin in combination with 5-fluorouracil (5-FU) in inhibiting the proliferation of MCF-7 cells.
METHODSA siRNA targeted to survivin was synthesized and transfected into MCF-7 cells via lipofectin. Changes of the cell growth activity in response to combined treatment with survivin siRNA and 5-FU or 5-FU treatment alone was evaluated by MTT assay. The Q method of Jin Zhenjun was used to evaluated synergism between the synthesized siRNA and 5-FU.
RESULTSTreatment with 5 nmol/L siRNA reduced the IC50 of 5-FU from 4.42 to 1.18 microg/ml, and the inhibitory effect of combined treatment on MCF-7 cells was higher than that of 5-FU alone (F=26.74, P<0.01). Synergism effect was observed between 5-FU at lower concentrations and survivin siRNA.
CONCLUSIONsiRNA may enhance the effectiveness of 5-FU in inhibiting the proliferation of MCF-7 cells.
