Synergism between a siRNA targeted to survivin and 5-FU in inhibiting MCF-7 cell proliferation in vitro
10.3321/j.issn:1673-4254.2006.03.001
- VernacularTitle:Survivin siRNA协同5-FU抑制MCF-7细胞的增殖
- Author:
Shu-Qun ZHANG
1
;
Shu-Hui ZHANG
;
Xin-Huan XUE
;
Xi-Jing WANG
;
Jian-Tao JIANG
Author Information
1. 西安交通大学附属第二医院
- Keywords:
RNA interference;
chemotherapy;
combined-modality therapy;
MCF-7 cells;
breast cancer
- From:
Journal of Southern Medical University
2006;26(3):251-254
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of small interfering RNA (siRNA) targeted to survivin in combination with 5-fluorouracil (5-FU) in inhibiting the proliferation of MCF-7 cells. Methods A siRNA targeted to survivin was synthesized and transfected into MCF-7 cells via lipofectin. Changes of the cell growth activity in response to combined treatment with survivin siRNA and 5-FU or 5-FU treatment alone was evaluated by MTT assay. The Q method of Jin Zhenjun was used to evaluated synergism between the synthesized siRNA and 5-FU. Results Treatment with 5 nmol/L siRNA reduced the IC50 of 5-FU from 4.42 to 1.18 μg/ml, and the inhibitory effect of combined treatment on MCF-7 cells was higher than that of 5-FU alone (F=26.74, P＜0.01). Synergism effect (Q ≥ 1.15) was observed between 5-FU at lower concentrations and survivin siRNA. Conclusion siRNA may enhance the effectiveness of 5-FU in inhibiting the proliferation of MCF-7 cells.
