Effect of photodynamic therapy with 5-aminolevulinic acid on human gastric cancer cells in vitro
10.3321/j.issn:1673-4254.2006.03.002
- VernacularTitle:5-氨基乙酰丙酸介导的光动力学治疗胃癌的实验研究
- Author:
Zong-Hai HUANG
1
;
Guang-Jun ZHOU
;
Jin-Long YU
;
Zhou LI
;
Lian-Shu DING
;
Ru-Xiang XU
;
Xiao-Dan JIANG
Author Information
1. 南方医科大学附属珠江医院
- Keywords:
gastric cancer;
5-aminolevulinic acid;
photodynamic therapy;
MGC-803 cells
- From:
Journal of Southern Medical University
2006;26(3):255-257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of 5-aminolevulinic acid (5-ALA)-mediated photodynamic therapy (PDT) on MGC-803 human gastric cancer cells in vitro. Methods MGC-803 human gastric cancer cells were treated with 5-ALA at various concentrations followed by laser irradiation. The cells were also treated with 5-ALA at the same concentration before laser exposure at various doses. PDT-induced phototoxicity of the cells was determined by MTT assay. Results After laser exposure of the cells at the same dose (25.0 J/cm2), the cell survival rates decreased significantly with incubation of the cells with 5-ALA at 0.25, 0.5, 1.0, 2.0 and 4.0 mmol/L, respectively (F=266.39, P＜0.001), but 2.0 and 4.0 mmol/L ALA showed no significant difference in lowering the cell survival rates (P＞0.05). Following treatment with the same 5-ALA concentration (1 mmol/L), the cell survival rates decreased in response to increased laser doses (at 6.25, 12.5, 25.0, 50.0, and 100 J/cm2, respectively, F=226.31, P＜0.0001). Without laser exposure, the survival rate of the cells did not significantly change for different 5-ALA concentrations (F=0.79, P=0.5383), nor did it undergo obvious variation in response to different laser doses without 5-ALA incubation (F=0.61, P=0.6551). Conclusions The damage of MGC-803 cells by PDT increases with 5-ALA concentration within a relative lower range and is proportional to the laser doses delivered. Without 5-ALA treatment, the laser at the chosen dose cannot produce photodynamic effect and ALA itself is nontoxic. ALA-mediated PDT appears to be a promising therapy for gastric cancer.
