Preparation of hematoporphyrin-herceptin photoimmunoconjugate for photoimmunotherapy.
- Author:
Lu CHEN
1
;
Rong-cheng LUO
;
Li-bo LI
;
Xiao YAN
;
Xue-mei DING
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Monoclonal; chemistry; pharmacology; Antibodies, Monoclonal, Humanized; Antineoplastic Agents; chemistry; pharmacology; Apoptosis; drug effects; Cell Line, Tumor; Cell Survival; drug effects; Drug Compounding; methods; Flow Cytometry; Hematoporphyrin Photoradiation; methods; Hematoporphyrins; chemistry; pharmacology; Humans; Immunoconjugates; chemistry; pharmacology; Immunotherapy; methods; Photosensitizing Agents; chemistry; pharmacology; Trastuzumab
- From: Journal of Southern Medical University 2006;26(3):355-357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare photoimmunoconjugate of hematoporphyrin (HP) and herceptin, and study its killing and apoptosis-inducing effect on tumor cells BT-474.
METHODSHP-herceptin photoimmunoconjugate was synthesized with EDCI as the condensator. After exposure of the cells to 630 nm laser, the killing effect of the conjugate and cell apoptosis were evaluated by MTT assay and flow cytometry.
RESULTSCompared with free HP at equivalent dose, the immune reactivity, killing effect and the apoptosis-inducing effect of HP-herceptin immunoconjugate on BT-474 cells was enhanced (P<0.05).
CONCLUSIONThe killing effect of HP-herceptin immunoconjugate is stronger than free HP on BT-474 cells.
