Effect of Bay K 8644 on arteriole smooth muscle cell membrane potential in rats with severe hemorrhagic shock.
- Author:
Qing ZHAO
1
;
Ke-sen ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: 3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester; pharmacology; Animals; Arterioles; physiopathology; Calcium Channel Agonists; pharmacology; Calcium Channels, L-Type; metabolism; Female; Male; Membrane Potentials; drug effects; Muscle, Smooth, Vascular; physiopathology; Potassium Channels, Calcium-Activated; metabolism; Rats; Rats, Wistar; Shock, Hemorrhagic; physiopathology
- From: Journal of Southern Medical University 2006;26(4):421-424
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Ca(2+) influx through L-type Ca(2+) channels on normal and hyperpolarized membrane potential of arteriole smooth muscle cells (ASMCs) in rats.
METHODSThe ASMCs isolated from normal rats and those with severe hemorrhagic shock were labeled with DiBAC4 (3) for membrane potential detection.
RESULTSCa(2+) influx caused hyperpolarization of the membrane potential in the normal ASMCs but depolarization in the cells from rats with hemorrhagic shock, and this effect could be inhibited by TEA.
CONCLUSIONCa(2+)-activated potassium channels activated by Ca(2+) influx through L-type Ca(2+) channels in normal ASMCs to cause hyperpolarization but leads directly to membrane potential depolarization in ASMCs from rats with severe hemorrhagic shock. This finding can be meaningful for treatment of vascular hyporeactivity in advanced stage of severe shock.
