Morphological changes of ECV304 cells infected by herpes simplex virus type 2.
- Author:
Hai-quan ZHAO
1
;
Wen-li MA
;
Ya-li ZHANG
;
Xiao-yang MO
;
Chang-wen KE
;
Huan-ying ZHENG
;
Wen-ling ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Endothelium, Vascular; pathology; virology; Herpesviridae Infections; pathology; Herpesvirus 2, Human; Humans; Necrosis; Umbilical Veins; pathology
- From: Journal of Southern Medical University 2006;26(4):445-447
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the pathological changes and morphological alterations of ECV304 cells after the infection by herpes simplex virus type 2 (HSV-2) in vitro.
METHODSPassaged ECV304 cells were infected with HSV-2, TCID50 and morphological changes were observed by optical microscopy and tissue staining.
RESULTSOne day after HSV-2 infection, swelling, rounding, and increase of thickened cytoplasmic granules occurred in the ECV304 cells, and on day 2, cell fusion was observed with weakened nuclear staining.
CONCLUSIONECV304 cells mostly undergo necrosis after HSV-2 infection without obvious evidence of cell apoptosis.
