Detection of mdr1 gene by real-time fluorescence quantitative polymerase chain reaction using Taq Man-MGB probe.
- Author:
Ya-wei ZOU
1
;
Zhi-chun FENG
;
Bin HU
;
Ying-sa QIAO
;
Zi-liang WU
;
Fu-xiong CHEN
;
Tie-zhen YE
Author Information
1. Department of Pediatrics, First Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, China. zouyawei@163.com
- Publication Type:Journal Article
- MeSH:
ATP-Binding Cassette, Sub-Family B, Member 1;
analysis;
genetics;
DNA Primers;
Female;
Fluorescent Dyes;
Fluorometry;
methods;
Genes, MDR;
genetics;
Humans;
Male;
Polymerase Chain Reaction;
methods;
Taq Polymerase
- From:
Journal of Southern Medical University
2006;26(4):466-468
- CountryChina
- Language:Chinese
-
Abstract:
Primer Express 2.0 software was used to design the primers and the MGB probe. With the plasmid pHaMDR1/A containing mdr1 cDNA as the template, we established a real-time fluorescent quantitative polymerase chain reaction system, which, at the template concentration of 3.061 x 10(3) to 3.061 x 10(9) cps/ml, had a correlation coefficient of 0.988243 between template concentration and threshold cycle value. This PCR method allows sensitive, specific and quantitative detection of human mdr1 gene.
