Sodium transport and its regulation of acutely isolated alveolar type II cells.
- Author:
Hai-yan SHEN
1
;
Tao-ping LI
Author Information
- Publication Type:Journal Article
- MeSH: Amiloride; pharmacology; Animals; Biological Transport; Cell Separation; Patch-Clamp Techniques; Pulmonary Alveoli; cytology; metabolism; Rats; Rats, Sprague-Dawley; Sodium; metabolism; Sodium Channel Blockers; pharmacology; Sodium Channels; metabolism; physiology; Terbutaline; pharmacology
- From: Journal of Southern Medical University 2006;26(4):476-478
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the sodium current of acutely isolated alveolar type II cells and its regulation.
METHODSAfter isolation the AT II cells, the whole cell sodium current were recorded in 5 hours with patch-clamp in whole-cell mode, and the effect of amiloride and terbutaline on the current was investigated.
RESULTSThe amiloride-sensitive current of AT II cells were recorded, which could be obviously stimulated by terbutaline.
CONCLUSIONAcutely isolated AT II cells can be valuable for studying electrophysiological characteristics of sodium channels even under pathological conditions.
