Effect of estrogen on osteoprotegerin, osteoclast differentiation factor and macrophage colony stimulating factor mRNA expressions in ovariectomized rat bone tissue.
- Author:
Qiang WANG
1
;
Kun-zheng WANG
;
Xiao-qian DANG
;
Zhi-bin SHI
;
Xian-wu PEI
;
Chuan-yi BAI
;
Xue-wu JIA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone and Bones; metabolism; Estrogens; pharmacology; therapeutic use; Female; Macrophage Colony-Stimulating Factor; biosynthesis; genetics; Osteoporosis; drug therapy; Osteoprotegerin; biosynthesis; genetics; Ovariectomy; RANK Ligand; biosynthesis; genetics; RNA, Messenger; biosynthesis; genetics; Rats; Rats, Sprague-Dawley
- From: Journal of Southern Medical University 2006;26(4):532-534
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effect of the estrogen on the mRNA expression of osteoprotegerin (OPG), osteoclast differentiation factor (ODF) and macrophage colony stimulating factor (M-CSF) in bone tissue of ovariectomized rats, and investigate the possible pathway of estrogen in preventing and treating postmenopausal osteoporosis. METHODS; Thirty healthy adult SD rats were randomly divided into sham operation group, ovariectomized group and estrogen-treated group. All rats were ovariectomized except those in the sham operation group. Bone density of the L3-L6 vertebra was detected 12 weeks after the operation. The total RNA were extracted from the femur to examine mRNA expression of OPG, ODF and M-CSF by real-time PCR.
RESULTSEstrogen increased the bone density of the ovariectomized rat lumbar vertebra and up-regulated the expression of OPG, whereas down-regulated the expression of M-CSF and lowered ODF:OPG ratio.
CONCLUSIONThe effect of estrogen in treating postmenopausal osteoporosis is closely correlated with the regulation of OPG and M-CSF expressions and ODF:OPG ratio.
