Effect of advanced oxidation protein products on nitric oxide production in mouse peritoneal macrophages.
- Author:
Zhong-hai LI
1
;
Shang-xi LIU
;
Fan-fan HOU
;
Yan-qun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Survival; drug effects; Cells, Cultured; Culture Media; chemistry; pharmacology; Dose-Response Relationship, Drug; Glycation End Products, Advanced; chemistry; Lipopolysaccharides; chemistry; pharmacology; Macrophages, Peritoneal; cytology; drug effects; metabolism; Mice; Nitric Oxide; biosynthesis; Oxidation-Reduction; Oxidative Stress; Reactive Oxygen Species; metabolism; Serum Albumin, Bovine; chemistry; pharmacology; Time Factors
- From: Journal of Southern Medical University 2006;26(5):558-560
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of advanced oxidation protein products (AOPP) on nitric oxide (NO) production in mouse peritoneal macrophages (MPMs).
METHODSMPMs were incubated in the absence or presence of lipopolysaccharide (LPS) with AOPP-modified bovine serum albumin (BSA) prepared by exposure of BSA to hypoclorous acid or pre-treated with AOPP-BSA and subsequent stimulation with LPS. NO production in the supernatants of the culture media was determined spectrophotometrically using Griess method. The cell viability was measured by MTT assay.
RESULTSBSA induced significant NO production in MPMs. AOPP modification of BSA significantly inhibited NO production, and AOPP-BSA exhibited time- and dose-dependent inhibition of NO production induced by LPS in MPMs incubated together with LPS or pre-treated before LPS stimulation.
CONCLUSIONAOPP-BSA is capable of inhibiting inducible NO production in MPMs.
