Differentiation of transplanted mouse c-Kit+lin- bone marrow cells into hepatocytes in vitro.
- Author:
Hao TANG
1
;
Cai-xian LIAO
;
Jie ZHOU
;
Hao-sheng JIN
;
Yuan-fei TAN
;
Jun SU
;
Chun-xing ZHANG
;
Shou-hua ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Bone Marrow Transplantation; methods; Cell Differentiation; Female; Hepatocytes; cytology; metabolism; Immunohistochemistry; Male; Mice; Mice, Inbred BALB C; Multipotent Stem Cells; metabolism; transplantation; Proto-Oncogene Proteins c-kit; metabolism; Random Allocation; Whole-Body Irradiation; alpha-Fetoproteins; metabolism
- From: Journal of Southern Medical University 2006;26(5):567-569
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo ascertain whether mouse c-Kit(+)Lin- bone marrow cells have the potential of hepatic stem cells.
METHODSc-Kit(+)lin- bone marrow cells were isolated and purified by magnetic-activated cell sorting (MACS) from BALB/C male donor mice, and immediately transplanted into age-matched BALB/C syngeneic female mice with 35-Gy total liver irradiation. The recipients were sacrificed 1 month after the transplantation for pathological observation of the liver morphology. The presence of Y-chromosome was examined in the liver cells of the recipient by in situ hybridization (ISH), and alpha-fetoprotein (AFP) and albumin in the cells were detected by immunohistochemistry.
RESULTSThe hepatocytes positive for Sry gene on Y-chromosome were identified 1 month after transplantation, and immunohistochemistry for AFP and albumin confirmed that the donor mice-derived cells were hepatocytes.
CONCLUSIONc-Kit(+)lin- bone marrow cells have the potential of hepatic stem cells, which can reside and differentiate into hepatocytes in the liver after transplantation. c-Kit(+)lin- bone marrow cells can be used as the source cells of cell transplantation for liver disease.
