Vector-mediated HER-2 RNA interference against HER-2-positive breast cancer.
- Author:
Xiao-qu HU
1
;
Li QIN
;
Feng-xi SU
;
He-rui YAO
;
Ji-sheng CHEN
;
Chang GONG
;
Ju-jiang GUO
;
Feng-yan YU
;
Hai-xia JIA
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Blotting, Western; Breast Neoplasms; genetics; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; Female; Genetic Vectors; Humans; RNA Interference; RNA, Messenger; genetics; metabolism; RNA, Small Interfering; genetics; Receptor, ErbB-2; genetics; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Transfection
- From: Journal of Southern Medical University 2006;26(5):570-572
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the feasibility of vector-mediated RNA interference for HER-2-positive breast cancer therapy.
METHODSA plasmid vector capable of mediating HER-2 RNA interference was constructed, and HER-2-positive breast cancer cell line SKBR-3 was transfected with this constructed vector. The expression of HER-2 mRNA and protein was analyzed by RT-PCR and Western blotting, and the growth and apoptosis of SKBR-3 cells was analyzed after transfection.
RESULTSThe expressions of HER-2 mRNA and HER-2 protein was downregulated in response to vector-mediated HER-2 RNA interference, which also resulted in tumor cell growth inhibition and increased number apoptotic cells.
CONCLUSIONHER-2 is a good target for RNA interference and RNA interference targeting HER-2 can lead to HER-2 breast cancer cell apoptosis and growth inhibition.
