Protective effect of grape seed proanthocyanidin on cultured RGC-5 cells against CoCl2-induced hypoxic injury.
- Author:
Ka-Na LIN
1
,
2
;
Mei-Li LIN
3
;
Er-Qing WEI
4
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Caspase 3; metabolism; Caspase 9; metabolism; Cell Hypoxia; drug effects; Cell Line; drug effects; Cell Survival; Cobalt; Down-Regulation; Grape Seed Extract; pharmacology; Proanthocyanidins; pharmacology; Proto-Oncogene Proteins c-bcl-2; metabolism; Reactive Oxygen Species; metabolism; Up-Regulation
- From: Journal of Zhejiang University. Medical sciences 2015;44(1):24-29
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the protective effects of grape seed proanthocyanidin extracts (GSPE) against CoCl2-induced hypoxic injury in cultured RGC-5 cells.
METHODSCoCl2(400 μmol/L) was used to induce hypoxic injury in cultured RGC-5 cells; the cells were pretreated with 0,100,200,400 and 800μmol/L GSPE for 24h. The cell viability was assayed by MTT; the apoptosis was detected by Hoechst 33342 staining; the intracellular reactive oxygen species (ROS) was measured by H2DCFDA oxidative reaction. The mRNA expression of Bcl-2, caspase 9 and caspase 3 was determined by real-time PCR.
RESULTSCompared to hypoxic control group, pretreatment with GSPE significantly increased viability of RGC-5 cells (P<0.001), reduced cell apoptosis (P<0 .001) and intracellular ROS(P <0 .001). In addition, GSPE significantly increased the mRNA expression of Bcl-2(P<0 .001) and decreased mRNA expression of caspase 9(P<0 .001) and caspase 3(P<0 .001) compared to hypoxic control group.
CONCLUSIONGSPE may have a protective effect against CoCl2-induced hypoxic injury in cultured RGC-5 cells. The decrease of intercellular ROS, up-regulation of Bcl-2 and down-regulation of caspase 9 and caspase 3 may be involved in the mechanism of the protective effect of GSPE.
