Effect of irradiated human lung fibroblasts on activation of canonical Wnt/β-catenin signaling pathway in mesenchymal stem cells.
- Author:
Chun-yang ZHANG
1
,
2
;
Yan ZHU
3
;
Hua-song FENG
3
;
Xu-xin CHEN
3
Author Information
- Publication Type:Journal Article
- MeSH: CCN Intercellular Signaling Proteins; metabolism; Cells, Cultured; Coculture Techniques; Fibroblasts; cytology; radiation effects; Gamma Rays; Glycogen Synthase Kinase 3; metabolism; Glycogen Synthase Kinase 3 beta; Humans; Intracellular Signaling Peptides and Proteins; metabolism; Mesenchymal Stromal Cells; metabolism; Proto-Oncogene Proteins; metabolism; Umbilical Cord; cytology; Wnt Signaling Pathway; X-Rays; beta Catenin; metabolism
- From: Journal of Zhejiang University. Medical sciences 2015;44(2):162-166
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of irradiated human lung fibroblasts (HLFs) on the canonical Wnt/β-catenin signaling pathway in human umbilical cord mesenchymal stem cells (HUMSCs).
METHODSHUMSCs were cultured alone (single group) or co-cultured with HLFs exposed to 5Gy X-rays (co-culture group). The protein levels of GSK-3β, p-GSK-3β, FRAT1 and β-catenin in HUMSCs were examined by Western blotting 3 days after culture or co-culture. WISP-1 protein levels in conditioned medium were examined by ELISA.
RESULTSThe levels of p-GSK3β/GSK3β (0.15 ± 0.05), FRAT1 (0.48 ± 0.07) and β-catenin (0.50 ± 0.07) in co-cultured HUMSCs significantly decreased compared to those in single group (0.55 ± 0.05, 1.16 ± 0.13 and 2.39 ± 0.15, all P<0.05). The supernatant level of WISP-1 in co-culture group was significantly decreased [(602.23 ± 161.47) ng/mL], compared to the single group [(977.77 ± 110.56) ng/mL, P<0.05].
CONCLUSIONIrradiated HLFs attenuate the activation of canonical Wnt/β-catenin signaling pathway in HUMSCs in vitro.
