Expression of S100A8 and A100A9 in giant cell tumor of bone and its relation with CT and MR imaging findings.

Jin-sheng Liao; Xiao-yi Ding; Shun-liang XU

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Expression of S100A8 and A100A9 in giant cell tumor of bone and its relation with CT and MR imaging findings.

Author: Jin-sheng LIAO ^{1
,

2} ; Xiao-yi DING ¹ ; Shun-liang XU ¹ ;
Author Information

1. Department of Radiology, the First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China
2. 2. Department of Radiology, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China.
Publication Type:Journal Article
MeSH: Bone Neoplasms; metabolism; Calgranulin A; metabolism; Calgranulin B; metabolism; China; Giant Cell Tumor of Bone; metabolism; Humans; RNA, Messenger; Tomography, X-Ray Computed; Up-Regulation
From: Journal of Zhejiang University. Medical sciences 2015;44(3):329-334
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the mRNA and protein expression levels of S100A8 and S100A9 in giant cell tumor (GCT) of bone, and its relation with radiological findings and biological behavior.
METHODSForty three patient with GCT of bone admitted in Ruijin Hospital Shanghai Jiaotong University School of Medicine from January 2009 to June 2012 were enrolled in the study. The expression levels of S100A8 and S100A9 mRNA and protein were detected by using semiquantitative RT-PCR and Western blotting in 43 specimens of GCT and 6 specimens of normal bone marrow. The CT and MRI findings of patients were retrospectively reviewed, its relation with tissue expression of S100A8 and S100A9 was analyzed.
RESULTSAmong 43 GCT cases 40 showed positive expression of S100A8 and S100A9 mRNA and protein, and the expression levels were significantly higher than those in normal bone marrow P<0.05). The expression level of S100A8 protein was significantly different in bone GCT with different composition ratio on MRI (P<0.05).The expression level of S100A9 protein was significantly different in GCT with different degree of bone destruction on CT scan (P<0.05).
CONCLUSIONThe expression of S100A8 and S100A9 mRNA and protein is up-regulated in GCT of bone. The expression of S100A8 and S100A9 is associated with the real composition ratio and the degree of bone destruction, respectively, indicating that S100A8 and S100A9 may be involved in the biological behavior of bone GCT.