Combination of lapatinib with chlorogenic acid inhibits breast cancer metastasis by suppressing macrophage M2 polarization.
- Author:
Jie-qiong ZHANG
1
;
Zhang-ting YAO
1
;
Gui-kai LIANG
1
;
Xi CHEN
1
;
Hong-hai WU
1
;
Lu JIN
2
;
Ling DING
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Chlorogenic Acid; pharmacology; Female; Lung Neoplasms; drug therapy; secondary; Macrophages; drug effects; Mammary Neoplasms, Experimental; drug therapy; Mice; Neoplasm Metastasis; drug therapy; Quinazolines; pharmacology
- From: Journal of Zhejiang University. Medical sciences 2015;44(5):493-499
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the effect of the combination of lapatinib with chlorogenic acid on metastasis of breast cancer in mouse model.
METHODSThe classical macrophage M2 polarization model induced by interlukin13in vitro was adopted in the study. Flow cytometric analysis was performed to detect the expression of M2 marker CD206. The transcription of M2-associated genes was measured by RT-PCR. HE staining was used to analyze the metastatic nodes of breast cancer in lungs of MMTV-PyVT mice. Immunostaining analysis was used to detect the expression of related proteins in breast cancer.
RESULTSThe combination of lapatinib and chlorogenic acid inhibited the expression of CD206 induced by IL-13[(42.17%±2.59%) vs (61.15%±7.58%), P<0.05]. The combination more markedly suppressed expression of M2-associated gene Ym1 than lapatinib alone[(0.9±0.1) vs (1.8±0.0), P<0.05]. The combination of lapatinib and chlorogenic acid significantly reduced metastatic nodes in lung[P<0.05], and also significantly decreased the percentage of CD206(+) cells in breast cancer compared to controls[(6.08%±2.60%) vs(29.04%±5.86%), P<0.05].
CONCLUSIONThe combination of lapatinib and chlorogenic acid can effectively inhibit macrophage M2 polarization and metastasis of breast cancer.
