Effect of poly(ADP-ribose)polymerase inhibitor combined with carbo on apoptosis of human breast cancer cells.
- Author:
Quan-wei TAO
1
;
Xiang-yang XIA
1
;
Qun-chao MA
1
;
Bo YANG
2
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Benzimidazoles; pharmacology; Breast Neoplasms; pathology; Carboplatin; pharmacology; Caspase 3; metabolism; Cell Line, Tumor; drug effects; Cell Survival; Humans; Poly(ADP-ribose) Polymerase Inhibitors; pharmacology; Proto-Oncogene Proteins c-bcl-2; metabolism; bcl-2-Associated X Protein; metabolism
- From: Journal of Zhejiang University. Medical sciences 2015;44(5):506-510
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of poly(ADP-ribose)polymerase(PARP)inhibitor ABT888 combined with carbo on apoptosis of human breast cancer cells.
METHODSMTT was used to detect the cell viability of MDA-MB-435s cells after treatment of carbo and ABT888 with different concentration. FACS and Western-blotting were used to detect the cell apoptosis rate and apoptosis-related protein expression, respectively.
RESULTSCombined application of carbo and ABT888 significantly inhibited the proliferation of MDA-MB-435s cells, and the inhibition rates were significantly higher than that of carbo or ABT888 alone. The combination of carbo and ABT888 markedly induced cell apoptosis(26.3%±1.5%) more than carbo(18.6%±1.6%, P<0.01) and ABT888(14.7%±2.3%, P<0.01) alone. Combination of carbo and ABT888 significantly down-regulated the expression of anti-apoptosis factors Bcl-2 and up-regulated the pro-apoptosis proteins Bax and cleaved caspase-3.
CONCLUSIONThe combination of carbo and ABT888 can suppress the proliferation and induce apoptosis of human breast cancer DA-MB-435s cells.
