Leptin as a survival enhanced-factor in grainy fat transplantation.
- Author:
You-bin WANG
1
;
Ke-ming QI
;
Min ZHAO
;
Qiang ZHUANG
Author Information
- Publication Type:Journal Article
- MeSH: Adipose Tissue; transplantation; Animals; Female; Humans; Leptin; pharmacology; Male; Models, Animal; Neovascularization, Physiologic; drug effects; Rats; Rats, Wistar; Survival Analysis; Time Factors; Transplantation, Homologous; Vascular Endothelial Growth Factor A; metabolism
- From: Chinese Journal of Plastic Surgery 2004;20(5):366-368
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThis experiment is to test the survival enhance effect of leptin in grainy fat transplantation.
METHODSThe 0.6 g grainy fat was obtained from the Wistar mouse subcutaneous tissue in the abdomen. It was then mixed with 0.9% NS and 20 ng/ml leptin and transplanted in the head subcutaneously. The transplanted tissue was taken out after 10, 20 and 40 days. After being weighed, the tissue was dyed with HE staining and VEGF staining. The data was analyzed with SPSS software.
RESULTSThe fibrous membrane around the tissue in leptin group was thin and there was less fat cell necrosis, compared with the control group. The remained-weight rate was 108.3%, 83.3% and 66.3% in 10, 20 and 40 days after the surgery. The differences were statistic significant between the leptin groups and the control (P < 0.05). The examinations of the HE and VEGF stain showed that there was the rich vascularization in leptin treated tissue.
CONCLUSIONLeptin could enhance the survival of the transplanted grainy fat due to its angiogenesis effect.
