Effect of expression of platelet-derived growth factor B gene on blood vessel reconstruction after tissue engineering skin grafting.
- Author:
Qian TAN
1
;
Xi CHEN
;
Zhi-wei LIANG
;
Pei-lin HUANG
;
Hong-reng ZHOU
;
Ding-wen YANG
;
Zi-hao LIN
;
Hua JIANG
Author Information
- Publication Type:Journal Article
- MeSH: Acellular Dermis; Animals; Male; Neovascularization, Physiologic; Proto-Oncogene Proteins c-sis; genetics; Rats; Rats, Sprague-Dawley; Skin; blood supply; Skin Transplantation; Skin, Artificial; Swine; Tissue Engineering; methods; Transfection
- From: Chinese Journal of Plastic Surgery 2004;20(6):447-450
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of PDGF on dermal blood vessel reconstruction by transplanted tissue-engineering skin containing PDGF-B gene to rats.
METHODSThe recombined eukaryotic expression vector, pcDNA3.1-hPDGF-B, was constructed and transfected into fibroblasts mediated by LipofectAMINE. Keratinocytes + acellular dermal matrix (group A), keratinocytes + acellular dermal matrix + fibroblasts (group B), keratinocytes + acellular dermal matrix + fibroblasts with PDGF gene (group C) were recombined respectively, then transplanted them to rat dorsum and evaluated the reconstruction of blood vessels in the dermis after 2, 4, 6 week postoperation.
RESULTSIn 2-4 weeks after skin grafting the vascularization rate in group C was higher than that of group B and group A. The vascularization rates in all groups had no significant differences in six weeks (P > 0.05).
CONCLUSIONPDGF-B gene plays an important role in reconstruction of blood vessels in the dermis at early tissue-engineering skin grafting, which ensures the take of grafted tissue-engineering skin.
