The effect of pcDNA 3.1/RPs15 on skin fibroblasts in vitro.
- Author:
Sheng-jian TANG
1
;
Xiao-ke WANG
;
Xiao-jie LV
;
Xia LIU
;
Peng ZHANG
;
Jun ZHANG
;
Xiao-yan ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; DNA Repair; DNA, Complementary; genetics; Epithelial Cells; metabolism; Fibroblasts; metabolism; Genetic Vectors; Mice; Nuclear Proteins; genetics; Plasmids; Ribosomal Proteins; Skin; cytology; Transfection
- From: Chinese Journal of Plastic Surgery 2005;21(1):58-61
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct eukaryotic expression vector of ribosomal protein sl5(RPs15) gene and study its effect on mouse skin fibroblasts in vitro.
METHODSThe RPs15 cDNA encoding region of fetal mouse skin was amplified by reverse transcription-polymerase chain reaction (RT-PCR) method and cloned into eukaryotic expression vector pcDNA3.1(-). The recombinant plasmid was transfected into adult mouse skin fibroblasts by FuGENE6 transfection reagents. Then the expression of RPs15 gene, was detected and its biological effect on fibroblasts was measured.
RESULTSThe DNA sequencing result of pcDNA3.1/RPs15 was identical with the reported. The RPs15 gene was expressed in transfected fibroblasts. The growth density of fibroblasts decreased with the conformation changing accordingly.
CONCLUSIONSThe eukaryotic expression vector pcDNA3.1/RPs15 is successively constructed and can be expressed in mouse skin fibroblasts. The results set up a basis for further study of the effect of RPs15 gene on skin fibroblasts.
