Experimental study on induction of the normal human melanocytes apoptosis in vitro by cycloheximide and TNF-alpha.

Author: Zhuang-Qun YANG ¹ ; Peng LI ; Jun-Bo TU ; Zheng-Hui WANG ; Xiao-Yi HU ; Tie-Liang ZHANG
Author Information

1. Department of Oral and Maxillofacial Plastic Surgery, Stomatological College, Xi'an Jiao Tong University, Xi'an 710004, China.
Publication Type:Journal Article
MeSH: Adolescent; Adult; Apoptosis; drug effects; Cell Proliferation; Cycloheximide; pharmacology; Drug Synergism; Humans; In Vitro Techniques; Male; Melanocytes; cytology; drug effects; Middle Aged; Tumor Necrosis Factor-alpha; pharmacology; Young Adult
From: Chinese Journal of Plastic Surgery 2005;21(4):302-305
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the effects of cycloheximide and TNF-alpha on melanocyte (MC).
METHODSMelanocyte apoptosis was studied with MTT, transmitting electron microscopy and fluorescence labeling of alive cells.
RESULTSWe added TNF-alpha and cycloheximide in melanocytes, and the typical apoptosis appeared 24 hours later, with chromatin condensation, nuclear pyknosis and apoptotic bodies formation. The results of cytometry showed the typical apoptotic peak.
CONCLUSIONTNF-alpha and cycloheximide together could inhibit MC proliferation and induce MC apoptosis.