Effects of exercise training on myocardial mitochondrial miR-499-CaN-Drp-1 apoptotic pathway in mice.
- Author:
Yong-cai ZHAO
- Publication Type:Journal Article
- MeSH: Animals; Apoptosis; Dynamins; metabolism; Heart; Male; Mice; Mice, Inbred C57BL; MicroRNAs; metabolism; Mitochondria, Heart; physiology; Myocardium; pathology; Physical Conditioning, Animal; Swimming
- From: Chinese Journal of Applied Physiology 2015;31(3):259-263
- CountryChina
- Language:Chinese
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Abstract:
OBJECTIVETo detect the levels of miR-499 and relative proteins in hearts of mice after exercise training, and investigate the mechanism of exercise-regulative apoptosis.
METHODSMale C57BL/6 mice were randomly divided into 3 groups( n = 14): sedentary (SE), exercise training 1 (ET1) and exercise training 2 (ET2) group. SE did not do any exercise. ET1 performed swimming training for 8 weeks. ET2 performed the same work as ET1 until the 5th week. Then, mice trained twice a day until the end of training. TUNEL assay was applied to test myocardial apoptosis, RT-PCR and Western blot were used to detect miR-499 and proteins levels respectively.
RESULTSCompared with SE, stress in ET1 failed to affect apoptotic index (AI) and miR-499-CaN-Drp-1 pathway (P > 0.05). In contrast, exercise load in ET2 increased miR-499 level, decreased Drp-1 level and AI with statistical significance respectively (P < 0.05), but neither CaN expression nor CaN activity was changed significantly (P > 0.05).
CONCLUSIONSwimming training can inhibit myocardial apoptosis, and the decrease in Drp-l may be responsible for the reduced myocardial apoptosis. CaN, the upstream protein, does not participate in exercise-regulative apoptosis.