The effects of Shadu Cao Mixture on immune functions of immunosuppression mice.
- Author:
Shun-fei LU
;
Liang-liang WANG
;
Jun-wan LU
;
Bing-jin LIU
;
Ying-peng TONG
;
Xiao-dong CHENG
;
Ting-mei YE
- Publication Type:Journal Article
- MeSH: Animals; Cyclophosphamide; Disease Models, Animal; Drugs, Chinese Herbal; pharmacology; Immunosuppression; Interferon-gamma; blood; Interleukin-2; blood; Killer Cells, Natural; immunology; Mice; Mice, Inbred BALB C; Spleen; immunology; Tumor Necrosis Factor-alpha; blood
- From: Chinese Journal of Applied Physiology 2015;31(3):266-269
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of Shadu Cao Mixture (SDCM, traditional Chinese medicine) on immune functions of immunosuppression mice.
METHODSFifty BALB/C mice were randomly divided into blank control group, model group, SDCM low-dose, middle-dose and high-dose group. Except the blank control group, other groups were intraperitoneal injected with cyclophosphamide (40 mg/kg) to establish immunosuppression mice model. The blank control group and model group received gavage administration with nonnal saline, while the other groups received gavage administration with different doses of SDCM (10, 20, 40 m/kg for 15 days) respectively. The number of leukocytes and serum levels of interleukin-2 (IL-2), tumor necrosis factor-α (TNF-α) and interferon-γ (IFN-γ) in peripheral blood, spleen index, and the function of NK cells were measured.
RESULTSCompared with the model group , SDCM increased the number of leukocytes and serum concentrations of IL-2, TNF-α and IFN-γ in peripheral blood and improved the spleen index and the function of NK cells significantly (P < 0.05-0.01).
CONCLUSIONSDCM could remarkably enhance the immune functions of immunosuppression mice induced by cyclophosphamide.