Effect of AAVC-I on the migration of human umbilical vein endothelial cells.
- Author:
Shu LI
;
Xin JIN
;
Xue-feng LONG
;
Jin-li JIA
;
Gen-bao ZHANG
;
Yun HONG
- Publication Type:Journal Article
- MeSH: Cell Movement; drug effects; Cells, Cultured; Crotalid Venoms; pharmacology; Down-Regulation; Human Umbilical Vein Endothelial Cells; drug effects; Humans; Intercellular Adhesion Molecule-1; metabolism; P-Selectin; metabolism; RNA, Messenger
- From: Chinese Journal of Applied Physiology 2015;31(5):407-410
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of component I from agkistrodon acutus venomon (AAVC-I) the migration of human umbilical vein endothelial cells (HUVECs), and to elucidate the possible anti-angiogenic mechanism of AAVC-I.
METHODSThe effect of AAVC-I on the migration of HUVECs which was cultivated in vitro and treated with AAVC-1 at four concentrations: 0, 20, 40, 80 microg/ml, was observed by methods of scratch wound-healing and Transwell assay. The expression level of mRNA and protein of P-selectin and intercellular cell adhension molecule-I (ICAM-1) were examined by RT-PCR and Western blot assay.
RESULTSCompared with the blank group, the migration ability of HUVECs in each AAVE-I treated group was reduced in a dose-dependent manner, and the expression level of the mRNA and protein of P-selectin and ICAM-1 were decreased.
CONCLUSIONAAVC-I inhibits the migration of endothelial cell, which is acted by down-regulation of the expression content of mRNA and protein of P-selectin and ICAM-1.