Exploring the active ingredient of Chinese yellow wine which could inhibit the Hcy induced proliferation and migration of vascular smooth muscle cells.
- Author:
Li-ping MENG
;
Chang-zuan ZHOU
;
Yan GUO
;
Xiao-ya ZHAI
;
Cheng-jian JIANG
;
Gang LI
;
Ju-fang CHI
;
Hang-yuan GUO
- Publication Type:Journal Article
- MeSH: Animals; Cell Movement; drug effects; Cell Proliferation; drug effects; Cells, Cultured; Homocysteine; Matrix Metalloproteinase 2; metabolism; Matrix Metalloproteinase 9; metabolism; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; cytology; drug effects; Peptides; chemistry; Polyphenols; chemistry; Rats; Tissue Inhibitor of Metalloproteinase-2; metabolism; Wine
- From: Chinese Journal of Applied Physiology 2015;31(5):437-442
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the active ingredients in the Chinese yellow wine could inhibit the proliferation and migration of rat vascular smooth muscle cells induced by homocysteine (Hcy).
METHODSThe primary culture and identification of rat vascular smooth muscle cells (VSMCs) was conducted, and the VSMCs in passage 4-7 were used in the following experiments. The VSMCs were divided into 7 groups: control, Hcy (1 mmol/L), Hcy + oligosaccharide, Hcy + polypeptides, Hcy + polyphenols, Hcy + alcohol, Hcy + Chinese yellow wine and were given the corresponding treatment. The proliferation of VSMCs was determined by MTT. Transwell chambers and would healing were employed to test the migratory ability of VSMCs. Wester blot and gelatin zymography were used to investigate the expressions and activities of metal matrix proteinase 2/9 (MMP-2/9) and tissue inhibitor of metalloproteinase 2 (TIMP-2) in VSMCs of each group.
RESULTSCompared with control group, the proliferation, migration and the expression and activity of MMP-2/9 of VSMCs were significantly increased in the VSMCs of Hcy group (P < 0.01). Compared with Hcy group, the proliferation, migration and the expression and activity of MMP-2/9 of VSMCs were significantly decreases in the VSMCs of polypeptides group, polyphenols group and Chinese yellow wine group. However, the expression of TIMP-2 among each group had no significant difference.
CONCLUSIONPolypeptides and polyphenols in the Chinese yellow wine could inhibit the proliferation and migration of VSMCs induced by Hcy.