Effects of inward rectifier potassium channel blockers on EPCs function.
- Author:
Wen-ping LI
;
Xiao-dong CUI
;
Ning-ning HOU
;
Xiao-yun ZHANG
;
Jian-hua LIU
;
Jing ZHANG
;
Min CHENG
- Publication Type:Journal Article
- MeSH: Animals; Barium Compounds; pharmacology; Cells, Cultured; Cesium; pharmacology; Chemokine CXCL12; metabolism; Chlorides; pharmacology; Endothelial Cells; cytology; Enzyme-Linked Immunosorbent Assay; Potassium Channels, Inwardly Rectifying; antagonists & inhibitors; physiology; Rats; Stem Cells; cytology
- From: Chinese Journal of Applied Physiology 2015;31(5):448-451
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of inward rectifier potassium channel blockers (BaCl2, CsCl) on the functions of endothelial progenitor cells (EPCs).
METHODSDensity gradient centrifugation-isolated rat hone marrow mononuclear cells were cultured in vitro. EPCs were harvested and seeded on six culture dish when cells grew to 3-5 passages. Before testing the EPCs were synchronized with M199, which contain 2% fetal calf serum. In the end, EPCs were treated with different intervention. The experiment mainly included two parts: (1) BaCl2 (100 micromol/L) and free BaC2 of Tyrodes solution; (2) CsCl (1 mmol/L) and control. Cell pretreated with blockers above mentioned for 12 h, then the gene expression of stromal cell-derived factor-1 (SDF-1), epoprotenol (PGI2) were assessed, beyond that the ability of adhesion, migration were assayed with different tests. In addition, the medium was collected when EPCs were treated for 3 days. The levels of SDF-1 were measured by sandwich enzyme-linked immunosorbent assay (ELISA). Going even further, EPCs were treated with the signal pathway blockers in advance, after repeat the above steps, in order to analyze the change of SDF-1 and then discuss its mechanism.
RESULTSCompared with control group, BaCl2, CsCl could increase EPC adhesion and migration to same extent. Moreover, the gene expression of SDF-1, PGI2 was significantly up-regulated and the production of SDF-1 increased evidently. Furthermore, the mechanism of SDF-1 secretion increasing mainly was associated with eNOS signaling pathways.
CONCLUSIONBa2+ and Cs+ play important roles in increasing EPCs functions, such as adhesion, migration and secretion.