The effects and mechanisms of high glucose on the phenotype transformation of rat vascular smooth muscle cells.
- Author:
Jing ZHANG
;
Hai-rong CHU
;
Ying GUO
;
Jian-hua LIU
;
Wen-Ping LI
;
Hong LI
;
Min CHENG
- Publication Type:Journal Article
- MeSH: Actins; metabolism; Animals; Aorta, Thoracic; cytology; Blotting, Western; Cells, Cultured; Glucose; pharmacology; Imidazoles; pharmacology; MAP Kinase Signaling System; Matrix Metalloproteinase 2; Matrix Metalloproteinase 9; metabolism; Muscle, Smooth, Vascular; cytology; Myocytes, Smooth Muscle; cytology; drug effects; Osteopontin; metabolism; Phenotype; Pyridines; pharmacology; Rats; p38 Mitogen-Activated Protein Kinases; metabolism
- From: Chinese Journal of Applied Physiology 2015;31(5):458-461
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects and mechanisms of high glucose on the phenotype transformation of rat vascular smooth muscle cells (VSMCs).
METHODSVSMCs ere isolated from rat thoracic aorta and the 3rd-5th VSMCs were incubated with normal glucose (5.5 mmol/L), high glucose (25 mmol/L), or high glucose (25 mmol/L) + P38 inhibitor (25 mmol/L +SB203580) for another 24 hours. Then the gene expression of osteopontin (OPN), alpha smooth-actin (alpha-SMA), matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9(MMP-9) were assayed by real time RT-PCR, the protein expression of P38 were assayed by Western blot.
RESULTS(1) High glucose promoted the phenotype transformation of VSMCs and up-regulated the expression of MMP-2 and MMP-9. (2) High glucose promoted the phosphorylation of P38. (3) SB203580, the inhibitor of P38/MAPK signal pathway, inhibited the effects of high glucose on phenotype transformation and expression of MMP-2 and MMP-9.
CONCLUSIONHigh glucose may promote phenotype transformation of VSMCs via the signal pathway of P38/MAPK.