The application of in vivo multi-channel recording methods in the studies of the affective pain in rats.
- Author:
Zhen-hua LI
;
Yang YANG
;
Miao-miao HOU
;
Yuan WANG
;
Xia QIN
;
Che ZHANG
;
Yu ZHANG
- Publication Type:Journal Article
- MeSH: Animals; Electrodes; Freund's Adjuvant; Gyrus Cinguli; cytology; Neurons; cytology; Pain; diagnosis; Pain Measurement; methods; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2016;32(1):41-45
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo record the electrical activities of Antirior cingulate cortex (ACC) neurons by in vivo multi-channel recording methods using the model of complete freund's adjuvant (CFA) induced conditioned place avoidance (C-CPA), which has been set up in our previous studies.
METHODSThe electrode was self-made and the CPA responses were recorded by in vivo multi-channel recording method.
RESULTS(1) The electrical activities of ACC neurons could be successfully recorded by the self-made electrode. (2) Before or after the injection of CFA, rats were respectively conditioned to the different place. The firing rates of ACC neurons in the CFA-paired place vs that in the non-CFA-paired place was (0.853 ± 1.377) imp/s vs (0.221 ± 0.971) imp/s (P < 0.05, n = 26). (3) The CPA responses in the CFA-paired place vs that in the non-CFA-paired place were (303.55 ± 61.77)s vs (140.32 ± 33.52)s(P < 0.05, n = 6).
CONCLUSIONThe firing rates of rACC (rostral Anterior Cingulate Cortex) neurons were involved in the occurrence of the affective pain.