Effect of total flavonoids of astragalus on endoplasmic reticulum chaperone, calumenin and connecxin 43 in suckling mouse myocardium with myocarditis caused by coxsackievirus B3.
- Author:
Li-ying XUAN
;
Xie-xin TAO
;
Ya-jun ZHAO
;
Hong-yan GE
;
Li-hong BAO
;
Da-peng WANG
;
Ming ZHAO
- Publication Type:Journal Article
- MeSH: Animals; Astragalus Plant; chemistry; Blotting, Western; Calcium-Binding Proteins; metabolism; Cells, Cultured; Connexin 43; metabolism; Coxsackievirus Infections; drug therapy; Endoplasmic Reticulum; metabolism; Endoplasmic Reticulum Stress; drug effects; Flavonoids; pharmacology; Heat-Shock Proteins; metabolism; Mice; Myocarditis; drug therapy; virology; Myocardium; cytology; Myocytes, Cardiac; drug effects; virology; Rats
- From: Chinese Journal of Applied Physiology 2016;32(1):51-54
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of total flavonoids of astragalus on the expression of endoplasmic reticulum chaperone, calumenin and connecxin 43 (CX43) in suckling mouse myocardium with myocarditis caused by coxsackievirus B3 (CVB3).
METHODSThe primary culture of suckling mouse myocardium cells were randomly divided into control group, CVB3 infected group and total flavonoids of astragalus group. Firstly, to confirm the identity of the suckling mouse myocardium, α-SMA was monitored by immunohistochemistry method. Then the protein expression changes of endoplasmic reticulum chaperone-glucose regulatory protein 78 ( GRP78), calumenin and CX43 were detected by Western blot.
RESULTS(1) Compared with that of the control group, the GRP78 expression level in CVB3 infected group was improved, the expression levels of calumenin and CX43 were all reduced. (2) Compared with that of CVB3 infected group, GRP78 expression level was decreased, and the expression levels of calumenin and CX43 were increased in total flavonoids of astragalus group.
CONCLUSIONCVB3 infection may cause endoplasmic reticulum stress of rat myocardium cells by increasing the expression of GRP78 and decreasing the expression of calumenin and CX43. On the other hand, total flavonoids of astragalus can reduce the expression of GRP78 and increase the expression of calumenin and CX43.The results of this experiment may be closely related to the effects of anti-arrhythmia with viral myocarditis caused by CVB3.