The influence of the aerobic endurance training on the skeletal muscular mitochondria function and PI3K-Akt protein expression.
- Author:
Shao-dong LIU
;
Yan-qiu ZHANG
;
Jiang CAO
- Publication Type:Journal Article
- MeSH: Animals; Electron Transport Complex IV; metabolism; Membrane Potential, Mitochondrial; Mitochondria; physiology; Muscle, Skeletal; physiology; Phosphatidylinositol 3-Kinases; metabolism; Physical Conditioning, Animal; Proto-Oncogene Proteins c-akt; metabolism; Rats; Signal Transduction; Succinate Dehydrogenase; metabolism
- From: Chinese Journal of Applied Physiology 2016;32(1):55-58
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the role of phosphatidylinositol 3-kinase--protein kinase B (PI3K-Akt) signaling pathway in the pro- tective effect of aerobic endurance training on the skeletal muscular mitochondria.
METHODSThirty-six rats were randomly divided into three groups( n = 12): control group, aerobic endurance training group and one-time exhaustive group. After the intervention, the quadriceps femoris muscle sample was obtained to detect the mitochondrial membrane potential( MMP), the activities of succinate dehydrogenase (SDH) and cy- tochrome coxidase (COX), and the protein levels of p-PI3K and p-Akt.
RESULTSCompared with the control group, the levels of mitochondrial membrane potential, the activities of succinate dehydrogenase and cytochrome coxidase, and the protein levels of p-PI3K and p-Akt were all significantly decreased in the one-time exhaustive group (P < 0.05). However, all the above was partially reversed in the endurance training group (P < 0.05), and there was no obvious difference with the control group (P > 0.05).
CONCLUSIONAerobic endurance training plays an important role in the protective effect on the skeletal muscular mitochondria, the mechanism may be related to activation PI3K-Akt signaling pathway.