Study on the changes of the leptin receptor expression and neuronal damage following focal ischemia/reperfusion in rats.
- Author:
Ying-Xue NIE
1
;
Hao TANG
;
Chuan-Sheng ZHAO
;
Jun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Ischemia; genetics; metabolism; pathology; Cerebral Cortex; metabolism; pathology; Disease Models, Animal; Gene Expression; Male; Neurons; pathology; Rats; Rats, Wistar; Receptors, Leptin; metabolism; Reperfusion Injury; metabolism; pathology
- From:Chinese Journal of Applied Physiology 2005;21(4):433-436
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the expression of leptin receptor (OB-R) and neuronal damage following focal ischemia/reperfusion in rats.
METHODS20 adult male Wistar rats were divided into four groups randomly: sham-operated 24 h,72 h control group and ischemic/reperfusion 24 h, 72 h experiment group. Focal ischemia/reperfusion model was made with MCAO. Immunohistochemistry and immunoelectron microscope were used to observe the expression of OB-R of the cortex and neuronal damage.
RESULTSThe positive cells of OB-R were found in pyramidal cells of the parietal cortex, choroid plexus and blood vessel endothelium. Compared with sham-operated group, significant reduction of OB-R positive cells in the pyramidal cells was observed in the ischemia/reperfusion rats 24 hours after cerebral ischemia (P < 0.05). The positive cells of OB-R of sham-operated 72 h group reduced further (P < 0.01). Histochemistry and electron microscope showed neuronal damage in the core area of cerebral ischemia in the late period was more obvious than in the early period.
CONCLUSIONThe early and delayed ischemia/reperfusion neuronal damage were accompanied with reduction of OB-R expression. Thus, it is worth to study the effect of OB-R in cerebral ischemia.
