Establishment of heterologous expression model of hSERT in Xenopus laevis oocytes.
- Author:
Yi-Ying WANG
1
;
Zhu JIN
;
Ci-Zhen LI
;
Yuan-Mou LIU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Carrier Proteins; genetics; DNA, Complementary; genetics; Female; Gene Expression; Models, Animal; Oocytes; metabolism; RNA, Messenger; genetics; Serotonin; metabolism; Serotonin Plasma Membrane Transport Proteins; biosynthesis; genetics; Xenopus laevis
- From: Chinese Journal of Applied Physiology 2005;21(4):444-448
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo determine the feasibility of establishing the heterologous expression model of human- serotonin transporter(hSERT or 5-HTT).
METHODScRNA of SERT was transcribed from cDNA, which was cloned in the pOTV vector. Each oocyte of mature xenopus laevis was injected with transcribed cRNA in vivo and incubated at room temperature for 4-9 days. Recording the current induced by 5-HT with voltage clamp technique tested the function of the expressed 5-HT transporter.
RESULTSThe transporter current could be observed in Ringer's solution containing 5-HT, and the 5-HT induced current were concentration-dependent. Norepinephrine and dopamine could not induce the transporter current while the 5-HT induced current could be specifically inhibited by 5-HTT blocker, desipramine.
CONCLUSIONThe results demonstrate that the heterologous expression product in xenopus laevis oocytes is human 5-HT transporter.
