The influence of acetylcholine on N receptor beta-subunit of the gastric epithelia and the gastric adenocarcinoma cells.
- Author:
Yan-Ping TANG
1
;
Jian-Guo WANG
Author Information
- Publication Type:Journal Article
- MeSH: Acetylcholine; pharmacology; Adenocarcinoma; metabolism; Cells, Cultured; Epithelial Cells; drug effects; Gastric Mucosa; cytology; Humans; Receptors, Nicotinic; metabolism; Stomach Neoplasms; metabolism; Tumor Cells, Cultured
- From: Chinese Journal of Applied Physiology 2005;21(4):457-460
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the influence of acetylcholine (ACh) on nicotinic receptor(N receptor) beta-subunit of the gastric epithelia and the gastric adenocarcinoma cells, and the difference of both cells.
METHODSImmunohistochemistry method was used to examine the number, number density and surface density of N receptor beta-subunit in both cells cultured in vitro.
RESULTSThe number and number density of N receptor beta-subunit in the gastric adenocarcinoma cells were much more than that in the gastric epithelia (P < 0.05). But surface density of N receptor beta-subunit in the gastric adenocarcinoma cells were lower than that in the gastric epithelia (P < 0.05). ACh at 10(6) mol/L could increase the number, number density and surface density of N receptor beta-subunit in the gastric epithelia (P < 0.01). The increase effect could not be blocked by atropine. ACh had no effect on N receptor beta-subunit in the gastric adenocarcinoma cells.
CONCLUSIONACh at low concentration initiates N receptor desensitization in the gastric epithelia. ACh has no effect on sensitivity of N receptor beta-subunit in the gastric adenocarcinoma cells.
