Effects and mechanisms of ginsenoside Rb3 on glutamate excitotoxic injury in cultured neurons of rat hippocampus.
- Author:
Hong-Mei SHEN
1
;
Zheng-Lin JIANG
;
Xiao-Song GU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cells, Cultured; Ginsenosides; pharmacology; Glutamic Acid; adverse effects; Hippocampus; cytology; drug effects; L-Lactate Dehydrogenase; metabolism; Neurons; drug effects; metabolism; Neuroprotective Agents; pharmacology; Nitric Oxide Synthase Type II; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2006;22(1):31-34
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo observe protective effects of ginsenoside Rb3 on glutamate excitotoxic injury in cultured hippocampal neurons and involved mechanisms.
METHODSOn cultured rat hippocampal neurons treated with glutamate at toxic concentration, we made the following investigations: by using MTT assay, LDH leakage detection, tests of total NOS, iNOS and cNOS activity, and the protective effects of ginsenoside Rb3.
RESULTSGinsenoside Rb3 can enhance the hippocampal neuronal viability, decrease the LDH leakage, elevate the viability of cNOS, and in the same time weaken iNOS's viability.
CONCLUSIONGinsenoside Rb3 has the significant protective effects on glutamate excitotoxic injury. The involved mechanism may include antagonizing the injury of neuron membrane, inhibiting the viability of iNOS, and increasing the activity of cNOS.
