Effect of vasonatrin peptide on the Ca2+ activated K+ channels of vascular smooth muscle cells isolated from rat mesentery arteries.
- Author:
Jun YU
1
;
Miao-Zhang ZHU
;
Li-Bing LIU
;
Bao-Ying CHEN
;
Shun-Yan LU
;
Jing-Jun ZHOU
;
Zhao-Jun FU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Atrial Natriuretic Factor; pharmacology; Male; Mesenteric Arteries; cytology; drug effects; metabolism; Muscle, Smooth, Vascular; metabolism; physiology; Potassium Channels, Calcium-Activated; drug effects; metabolism; Rats; Rats, Sprague-Dawley
- From: Chinese Journal of Applied Physiology 2006;22(1):94-97
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo investigate effect and mechanism of vasonatrin peptide (VNP) on Ca2+ activated K+ channels (K(Ca)) of vascular smooth muscle cells (VSMCs) isolated from rat mesentery arteries.
METHODSChanges of K(Ca) induced by VNP were measured by the means of whole cell recording mode of patch clamp, furthermore effects of HS-142-1(0.3 g/L), 8-Br-cGMP and methylene blue (MB) were observed.
RESULTSK(Ca) was significantly enhanced by VNP (10(-6) mol/L), which was mimicked by 8-Br-cGMP(10(-3) mol/L) and blocked completely by HS-142-1 or MB (2 x 10(-5) mol/L).
CONCLUSIONVNP increases K(Ca) of VSMCs isolated from rat mesenteric arteries, by binding with natriuretic peptide guanylate cyclase-coupled receptors and increasing the intracellular level of cGMP in VSMCs.
