Isolation, incubation and differentiation of pancreatic islet-derived progenitor cells from newborn SD rats.
- Author:
Qing-Yong WANG
1
;
Su-Fang LIU
;
Wei-Feng WANG
;
Ping CHEN
;
Yun-Wei YAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Animals, Newborn; Cell Culture Techniques; Cell Differentiation; drug effects; Cell Separation; Cells, Cultured; Glucagon-Like Peptide 1; pharmacology; Islets of Langerhans; cytology; Male; Rats; Stem Cells; cytology
- From: Chinese Journal of Applied Physiology 2006;22(1):125-128
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo isolate, culture pancreatic progenitor cells derived from islets of newborn rats, and to observe the effect of GLP-1 (7-36) NH2 on islet progenitor differentiation.
METHODSIslets were isolated purified, islet progenitor cells were isolated and proliferated in the modified RPMI-1640 medium supplemented with 20 microg/L bFGF and 20 microg/L EGF, then were differentiated with 20 nmol/L GLP-1 (7-36) NH2. The properties of islet progenitor cells were identified primarily by hybridization in situ, immunocytochemistry, dithizone (DTZ)-staining, and radioimmunoassay before and after differentiation.
RESULTSIslet progenitor cells did not express PDX-1, insulin and somatostatin, but nestin. After differentiation, a portion of cells expressing PDX-1, insulin mRNA, insulin, somatostatin, and nestin, islet-like cell clusters (ICCs) were formed, DTZ-stained cells were in peripheral region of it. Insulin release was markedly greater in media harvested after differentiation of 3 weeks.
CONCLUSIONA kind of progenitor cells exists in pancreatic islet of newborn SD rats, could be expanded continuously. GLP-1 (7-36) NH2 could differentiate pancreatic islet-derived progenitor cells to form ICCs capable of insulin secretion.
